2010 Quadrifoglio Verde (Cloverleaf in UK) version of Mito was presented in 2009 Frankfurt Motor Show. The Quadrifoglio Verde (English: green four-leaf clover) has traditionally been the highest line of Alfa Romeo models. The QV version had the 1.4-litre 170 PS (125 kW; 168 hp) Multiair engine and specially engineered suspension, steering and six-speed C635 gearbox developed by Fiat Powertrain Technologies (FPT). Multiair technology allowed fuel consumption of 6 litres per 100 kilometres (47 mpg-imp; 39 mpg-US) in EU combined driving and CO2 emissions of 139 g/km.

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2014 facelift brought a TCT Dual Dry Clutch Transmission to Quadrifoglio Verde MiTo while a manual version was no longer available.